Description
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Tumor necrosis factor receptor superfamily member EDAR concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor necrosis factor receptor superfamily member EDAR, During the reaction, Tumor necrosis factor receptor superfamily member EDAR in the sample or standard competes with a fixed amount of biotin-labeled Tumor necrosis factor receptor superfamily member EDAR for sites on a pre-coated Monoclonal antibody specific to Tumor necrosis factor receptor superfamily member EDAR. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Tumor necrosis factor receptor superfamily member EDAR in the samples is then determined by comparing the O.D. of the samples to the standard curve